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PhD Dissertation Defense: Shayan Borhani

Format: In-Person Presentation (with virtual option)

Location

Technology Research Center (TRC) : 206

Date & Time

June 13, 2023, 1:00 pm2:00 pm

Description

Shayan Borhani, Chemical and Biochemical Engineering Ph.D. Candidate


Advisor: 

Dr. Govind Rao

TITLE:

Alternative approaches to insulin manufacturing using cell-free systems

ABSTRACT: 

The modular nature of Cell-Free Protein Synthesis (CFPS) has resulted in a paradigm shift in the way scientists can design, discover, and manufacture therapeutic proteins. This dissertation reports on three advances made in point-of-care (POC) manufacturing of therapeutics by using a CFPS systems to address current challenges around pandemic preparedness and future therapeutic shortages. First, the utility of both a prokaryotic and eukaryotic cell-free systems (CFS) was exploited to synthesize human proinsulin to assess the extent of post translational modification and product quality achieved by each. Second, a “on-column” purification approach was developed for post-translational conversion of proinsulin into mature human insulin using a specialized set of proteases. Third, a technoeconomic model was utilized to access the cost effectiveness of cell-free manufacturing of insulin in comparison to the current state of the art. A less complex protein target, Griffithsin (GRFT), was also tested using similar approaches. 



Agenda
LOCATION: TRC 206 & WEBEX
12:45 pm EST - Welcome
1:00 pm EST - Presentation followed by questions from the audience. 
After the questions from the public, the meeting will be closed for the committee discussion.


WEBEX information: 
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