← Back to Event List

PhD Dissertation Defense: Zahra Ghassemi

Location

Online

Date & Time

April 8, 2022, 3:00 pm4:00 pm

Description

Zahra GhassemiPhD Candidate

Advisor:

Dr. Jennie Leach 

Protein confinement and effects on thermodynamic stability, aggregation, and enzymatic activity



Abstract:

Proteins are complex, vulnerable macromolecules susceptible to denaturation in biological and manufacturing processes. Nearly all proteins require their native structure to function properly. In vivo, proteins fold in environments composed of small molecules, macromolecules, and larger structures. The macromolecules in particular play unique roles influencing protein structure by presenting a 3D “confining” environment that limits protein mobility. However, most protein structure and aggregation studies are carried out in buffer solutions where proteins are "free" to diffuse. To mimic the in vivo scenario, crowding agents are added to the buffers; the term "crowded" refers to the presence of a high concentration of uncrosslinked macromolecules that exclude solvent volume available to proteins. These works have unveiled insights into protein structure during crowding; however, protein structure under confinement is yet poorly understood. 

Herein, a novel means to controllably crowd and confine proteins is presented: macromolecules crosslinked are into a hydrogel, forming fixed barriers or a “mesh”, confining the proteins, while simultaneously excluding solution volume due to the macromolecules themselves. This dissertation reports protein confinement effects on thermodynamic stability, aggregation kinetics, and enzymatic activity. Model proteins were confined within homogeneously-crosslinked hydrophilic poly(ethylene) glycol (PEG) hydrogels. Primary findings include: i) when hydrogel mesh size is approximate to protein size, a protein subset has enhanced stability (i.e., aggregation of hydrophilic proteins is decelerated or prevented, yet aggregation of hydrophobic proteins is promoted); ii) enzymatic activity is better preserved in PEG hydrogels than free buffer; and iii) an insightful generalized model correlating mesh size, protein unfolding free energy, and protein size that is sensitive to the number of amino acid residues, stability, aspect ratio, and accessible surface area. This work provides a novel means to influence protein stability to solve complex problems related to disease and therapeutics development. 

Agneda

2:45 AM EST - Virtual WEBEX room opens
3:00 PM EST - Presentation followed by questions from the audience. 
After the questions from the public, the meeting will be closed for the committee discussion.

WEBEX Information: 
use the 'Join Online Event' button above
Join by phone
+1-202-860-2110 United States Toll (Washington D.C.)
Access code: 2620 138 7875